LigandTracer Green

• Estimate the affinity and kinetics of interactions with living cells

• Use regular "100 mm" Petri dishes of any brand (Ø:87-89 mm and h: 15-18 mm without the lid)

• Measure at room temperature, in incubators, or in cold rooms

• Plan your run and store relevant information with our intuitive control software

• Estimate interaction parameters with our versatile evaluation tool TraceDrawer

• Quickly advance your knowledge with LigandTracer Learning

LigandTracer® monitors molecular interactions in real-time, right on your lab bench, with only a few preparatory steps and limited input. The most common target is a cellular receptor expressed on the surface of living cells, but almost any interaction can be monitored as long as:

• You can label your ligand of interest

• Targets can be attached to a Petri dish

• The interaction occurs within minutes to hours

Detects fluorescence. Seven interchangeable detectors available:

• BlueGreen: 488 nm – 535 nm (ex/em), e.g. FITC and Alexa Fluor™ 488

• BlueLime: 488 nm – 575 nm (ex/em), e.g. PE

• GreenOrange: 535 nm – 589 nm (ex/em), e.g Cy3 and Atto Rho6G

• OrangeRed: 589 nm – 632 nm (ex/em), e.g. Texas Red and Atto 590

• RedNIR: 632 nm – 676 nm (ex/em), e.g. Alexa Fluor™ 633 and Atto 647

• RedNIR+: 632 nm – 700 nm (ex/em), e.g. Alexa Fluor™ 680, IRDye® 680, and fluorophores suitable for RedNIR (632 nm/676 nm)

• NIR++IR: 740 nm – 810 nm (ex/em), e.g. Alexa Fluor™ 790 and IRDye® 800CW

LigandTracer Green is compatible with LigandTracer MultiDish 2×2, which allows you to run two separate experiments at identical conditions in parallel. Detector: Interchangeable LED-based fluorescence detector

• Drug discovery and development

• Antibody characterization

• Receptor–ligand interaction studies

• Affinity and kinetic analysis

• Receptor quantification

• Specificity and competition studies

• Receptor clustering and dimerization

• Drug conjugate characterization

• Radiotracer development

• Nanoparticle characterization

• Cell-based interaction studies

• Physiological process studies

• Screening and lead evaluation