Prometheus™ Panta

• Combines four analytical technologies in a single instrument: nanoDSF, DLS, SLS, and backreflection

• Provides both conformational and colloidal stability information in one experiment

• Measures multiple protein stability parameters from a single 10 µL sample

• Label-free analysis without dyes or additives

• Enables simultaneous characterization of thermal stability, aggregation, particle size, polydispersity, and molecular weight

• Suitable for protein formulation, biologics developability, structural biology, and biopharmaceutical research

• Supports automated workflows and high-throughput screening with the optional robotic autosampler

• Generates structured JSON data exports for integration with third-party software and analysis pipelines

Prometheus™ Panta is a multi-parameter protein stability characterization platform designed to provide comprehensive insights into the conformational and colloidal stability of proteins and biologics. The system combines nanoDSF, Dynamic Light Scattering (DLS), Static Light Scattering (SLS), and Backreflection to collect complementary information throughout a thermal ramp. This allows researchers to evaluate protein unfolding, aggregation, particle size, polydispersity, molecular weight, and self-association from a single sample. With a sample requirement of only 10 µL and label-free measurement, Prometheus Panta is well suited for biologics developability, formulation screening, membrane protein studies, structural biology, and CMC-related characterization.

Measurement technologies: nanoDSF, DLS, SLS, backreflection Sample volume: 10 µL Measurement principle: label-free optical analysis Temperature control range: 15–110°C Temperature control precision: ±0.1 °C DLS laser: 405 nm DLS laser: power 100 mW max. DLS parameters: hydrodynamic radius (rH), PDI, and aggregation-related parameters SLS parameters: Scattering intensity, molecular weight nanoDSF parameters: Tm, Tonset, unfolding-related parameters Backreflection: Turbidity / large aggregate detection Automation: Optional Robotic Autosampler Maximum automated throughput: up to 1,536 samples with a robotic autosampler Data export: Human- and machine-readable JSON Software: Prometheus Panta Control & Analysis software Operating temperature: 15–30°C Humidity 20–70%, non-condensing

• Protein stability characterization

• Biologics developability

• Thermal shift assays

• Protein formulation screening

• Pre-formulation and formulation development

• Aggregation and self-association studies

• Membrane protein characterization

• Structural biology

• Protein engineering and construct screening

• Antibody characterization

• Therapeutic protein development

• AAV and gene therapy vector characterization

• Protein-based vaccines and VLPs

• CMC analytical testing