Andromeda X

• Analyze protein expression and thermal stability in crude lysates

• No protein purification required before measurement

• Screen up to 48 samples in under 15 minutes

• Uses only 10 µL of lysate per measurement

• Highly sensitive spectral shift optics for detecting low-expression proteins

• Provides information on both protein expression level and folding/stability

• Perform in-lysate thermal shift assays to evaluate protein functionality and ligand binding

• Compatible with lysates from E. coli, HEK293, and Sf9 expression systems

• Can also analyze cell culture supernatants containing secreted proteins

• Site-specific labeling options are available for His-tagged, SNAP-tagged, biotinylated, and human Fc-tagged proteins

Andromeda X is a protein expression and stability analysis system designed to help researchers evaluate recombinant proteins before purification. By working directly with crude lysates, the system provides information on protein expression, folding, and thermal stability without requiring a purification step. Andromeda X uses highly sensitive Spectral Shift optics to selectively monitor the target protein during a temperature ramp. Changes in fluorescence are used to determine the inflection temperature (Ti), helping researchers compare expression conditions, identify stable protein constructs, and evaluate ligand-induced stabilization. With a low sample requirement of 10 µL and the ability to screen up to 48 samples in under 15 minutes, Andromeda X can help researchers identify promising protein constructs and expression conditions early in the workflow.

Measurement technology: Spectral Shift Sample type: Crude lysates, cell culture supernatants Sample volume: 10 µL per measurement Sample purification: Not required Maximum samples per run: 48 samples Measurement time: Less than 11 minutes for 48 samples at maximum heating rate Typical total workflow: About 15 minutes, including labeling and measurement Maximum heating rate: 7.0°C/min Main output: Inflection temperature (Ti), fluorescence signal / expression-related information Detection: Fluorescence at 650 nm and 670 nm Expression systems: E. coli, HEK293, Sf9 Labeling options: His-tag, SNAP-tag, biotinylated, human Fc Analysis software: AN.Control / AN. Stability Analysis

• Recombinant protein expression screening

• Protein stability analysis

• In-lysate thermal shift assays

• Protein construct screening

• Expression condition optimization

• Pre-purification quality assessment

• Protein folding analysis

• Ligand binding / functionality assessment

• Antibody fragment screening

• Membrane protein detergent screening

• Protein engineering

• Biopharmaceutical research

• Early-stage protein characterization