LSM Upgrade Kit
Compact, easy-to-use, and maintenance-free kit, customized to all major LSMs in various configurations for unlimited flexibility Highest sensitivity with up to 4 detection channels Fluorescence lifetimes from < 100 ps up to µs rapidFLIM, FLIM, FRET, and FCS in one turn-key system rapidFLIM HiRes—visualize dynamic processes with ultra-fast FLIM imaging and outstanding 5 ps time resolution
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Description
Confocal Laser Scanning Microscopes (LSMs) are widely used tools in biochemistry, cell biology, and other related life sciences. The capabilities of these microscopes can be further enhanced by using time-resolved techniques, granting the following advantages: Lifetime FRET for quantitative measurements of FRET efficiency Time-resolved imaging reveals environmental parameters such as ion concentrations or pH Lifetime measurements are independent of fluorophore concentration Separation of molecules with spectrally overlapping emission by lifetime fingerprinting Reduced number of needed detectors—one detector is sufficient for simultaneous detection of different fluorophores based on their specific lifetimes by pattern matching Discrimination of fluorescence light against elastic and Raman scattering and other background contributions by temporal resolution Decay time as a further parameter enhances the accuracy of analytical measurements The upgrade kit is an external addition to the LSM and grants easy-to-use, versatile, and comfortable performance without time-consuming adjustments. The turnkey setup consists of three major parts: picosecond pulsed excitation, single-molecule sensitive detection, and Time-Correlated Single Photon Counting (TCSPC) data acquisition.
Specifications
Excitation System Picosecond diode lasers with adjustable output power and repetition rates up to 80 MHz inside a compact laser combining unit Wavelengths between 375 and 900 nm Single- or multi-channel laser driver Optional: external laser (e.g., Titanium: Sapphire laser) New: 560 nm picosecond pulsed excitation with the LDH-D-TA-560 Supported LSMs Nikon: AX, A1, C2+, C2, C1si Olympus: FluoView, FV4000, FV3000, FVMPE-RS, FluoView FV1200 (MPE), FluoView FV1000 (MPE) Scientifica: VivoScope, HyperScope Zeiss: LSM 980, LSM 880, LSM 780, LSM 710 Detection Up to four parallel detection channels Descanned or non-descanned configuration Connection via optical fibers to the LSM Detectors Hybrid-Photomultiplier Tubes Single Photon Avalanche Diodes Photomultiplier Tubes Data acquisition Based on the method of Time-Correlated Single Photon Counting (TCSPC) in the unique Time-Tagged Time Resolved (TTTR) measurement mode Simultaneous data acquisition in up to four channels Software SymPhoTime 64 NovaFLIM
Application
Time-Resolved Fluorescence NEW – rapidFLIM HiRes—Redefining standards for dynamic FLIM imaging Fluorescence Lifetime Imaging (FLIM) Phosphorescence Lifetime Imaging (PLIM) Fluorescence Correlation Spectroscopy (FCS) Fluorescence Lifetime Correlation Spectroscopy (FLCS) Fluorescence Cross-Correlation Spectroscopy (FCCS) Foerster Resonance Energy Transfer (FRET) Pulsed Interleaved Excitation (PIE) Laser Cutting/Ablation Pattern Matching Analysis Time-Resolved Photoluminescence (TRPL) TRPL Imaging Antibunching Anisotropy