LSM Upgrade Kit
Compact, easy-to-use, and maintenance-free kit, customized to all major LSMs in various configurations for unlimited flexibility
Highest sensitivity with up to 4 detection channels
Fluorescence lifetimes from < 100 ps up to µs
rapidFLIM, FLIM, FRET, and FCS in one turn-key system
rapidFLIM HiRes—visualize dynamic processes with ultra-fast FLIM imaging and outstanding 5 ps time resolution
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Description
Confocal Laser Scanning Microscopes (LSMs) are widely used tools in biochemistry, cell biology, and other related life sciences. The capabilities of these microscopes can be further enhanced by using time-resolved techniques, granting the following advantages:
Lifetime FRET for quantitative measurements of FRET efficiency
Time-resolved imaging reveals environmental parameters such as ion concentrations or pH
Lifetime measurements are independent of fluorophore concentration
Separation of molecules with spectrally overlapping emission by lifetime fingerprinting
Reduced number of needed detectors—one detector is sufficient for simultaneous detection of different fluorophores based on their specific lifetimes by pattern matching
Discrimination of fluorescence light against elastic and Raman scattering and other background contributions by temporal resolution
Decay time as a further parameter enhances the accuracy of analytical measurements
The upgrade kit is an external addition to the LSM and grants easy-to-use, versatile, and comfortable performance without time-consuming adjustments. The turnkey setup consists of three major parts: picosecond pulsed excitation, single-molecule sensitive detection, and Time-Correlated Single Photon Counting (TCSPC) data acquisition.
Specifications
Excitation System
Picosecond diode lasers with adjustable output power and repetition rates up to 80 MHz inside a compact laser combining unit
Wavelengths between 375 and 900 nm
Single- or multi-channel laser driver
Optional: external laser (e.g., Titanium: Sapphire laser)
New: 560 nm picosecond pulsed excitation with the LDH-D-TA-560
Supported LSMs
Nikon: AX, A1, C2+, C2, C1si
Olympus: FluoView, FV4000, FV3000, FVMPE-RS, FluoView FV1200 (MPE), FluoView FV1000 (MPE)
Scientifica: VivoScope, HyperScope
Zeiss: LSM 980, LSM 880, LSM 780, LSM 710
Detection
Up to four parallel detection channels
Descanned or non-descanned configuration
Connection via optical fibers to the LSM
Detectors
Hybrid-Photomultiplier Tubes
Single Photon Avalanche Diodes
Photomultiplier Tubes
Data acquisition
Based on the method of Time-Correlated Single Photon Counting (TCSPC) in the unique Time-Tagged Time Resolved (TTTR) measurement mode
Simultaneous data acquisition in up to four channels
Software
SymPhoTime 64
NovaFLIM
Application
Time-Resolved Fluorescence
NEW – rapidFLIM HiRes—Redefining standards for dynamic FLIM imaging
Fluorescence Lifetime Imaging (FLIM)
Phosphorescence Lifetime Imaging (PLIM)
Fluorescence Correlation Spectroscopy (FCS)
Fluorescence Lifetime Correlation Spectroscopy (FLCS)
Fluorescence Cross-Correlation Spectroscopy (FCCS)
Foerster Resonance Energy Transfer (FRET)
Pulsed Interleaved Excitation (PIE)
Laser Cutting/Ablation
Pattern Matching Analysis
Time-Resolved Photoluminescence (TRPL)
TRPL Imaging
Antibunching
Anisotropy