Luminosa Fluorescence Microscopes
- Single-photon counting confocal microscope with intuitive, software-controlled operation. - Versatile excitation system (375–1064 nm lasers) for broad applications. - High-performance detection: up to 6 parallel channels with SPADs or hybrid PMTs. - One-click autoalignment and GPU-accelerated workflows for fast, reliable results. - Ideal for FLIM, FCS, and single-molecule studies with superior resolution and contrast.
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Description
Luminosa is an all-in-one fluorescence microscope that pairs the highest data quality with remarkably simple day-to-day operation. It easily integrates into any researcher’s toolbox. It becomes a time-efficient, reliable companion for scientists starting to explore time-resolved fluorescence methodologies as well as for experts wanting to push the limits. Truly a microscopy system that everybody can rely on.
Specifications
Excitation 1 - 8 lasers with choice of wavelengths from 375 - 1064 nm optional: 2 additional exit ports available for coupling additional lasers Commonly used wavelengths: 405, 440, 485, 532, 560, 595, and 640 nm Individual laser operation modes : pulsed and continuous wave (Continuous mode availability depends on the laser model.) Repetition rates :1 - 40 MHz, for specific wavelengths up to 80 MHz Laser driver :8 channel Sepia PDL 828L Motorized main dichroic wheel : 6 positions available for 18 × 25 × 3 mm single, dual, triple, quad-band dichroics Microscope inverted Olympus IX73 body (partially motorized) Objective types: UPLSAPO 60x PlanApochromat, NA 1.2, water immersion, 400 - 900 nm UPLSAPO 100x PlanApochromat, NA 1.4, oil immersion, 400 - 850 nm other oil immersion, apochromatic correction, air spaced, IR-enhanced, or long working distance objectives available Observation modes : confocal, transmission optional: epi-widefield illumination, Differential Interferometric Contrast (DIC) Oculars : Yes Camera : Optional CCD camera at the left side port Sample holder : Multi-functional sample holder compatible with several standard sizes of microscope coverslips and petri dishes 96-well plate sample holder Compatible with cage incubator : Yes Positional stability Axial drift (typical values) • For 15-minute measurement: 50 ± 10 nm • For 2-hour measurement: 100 ± 20 nm Point Detector Type • Single Photon Avalanche Diodes (SPAD) for low dark-counts and maximum detection efficiency • Hybrid photomultiplier tubes (PMA-Hybrid-40) for optimum timing performance and when working at high count rates • 23-pixel SPAD array Data acquisition • based on Time-Correlated Single Photon Counting (TCSPC) in Time- Tagged Time Resolved (TTTR) measurement mode • simultaneous data acquisition with up to 6 channels • new dynamic binning format for FLIM imaging TCSPC Unit : MultiHarp 150 8P Scanning and positioning FLIMbee : XY galvo scanner : Min. pixel dwell time: 0.5 µs, Min. pixel size: 17 nm (60x objective) Up to 5.2 frames per second for a 512 x 512 pixel image Max. scan field: 200 x 200 µm (60x objective) Possibility to bypass the FLIM bee galvo scanner for point measurements XY objective scanning : Min. pixel dwell time: 0.2 ms Min. pixel size: 1 nm (independent of objective) Max. scan field: 80 x 80 µm (independent of objective) Luminosa software Operating System : Windows 11 GPU-based programming OpenGL Context-based workflows for easy acquisition and analysis For single-molecule FRET burst analysis, single-molecule imaging, FCS in vitro, FCS in cells, FLIM, FLIM-FRET, steady-state FRET, anisotropy imaging
Application
-Dynamic structural biology at the single molecule level -Cellular mechanisms driven by phase separation -Environmental sensing -Mapping dynamics and structure of cellular membranes -Plant development and structure