Luminosa Fluorescence Microscopes
- Single-photon counting confocal microscope with intuitive, software-controlled operation.
- Versatile excitation system (375–1064 nm lasers) for broad applications.
- High-performance detection: up to 6 parallel channels with SPADs or hybrid PMTs.
- One-click autoalignment and GPU-accelerated workflows for fast, reliable results.
- Ideal for FLIM, FCS, and single-molecule studies with superior resolution and contrast.
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Description
Luminosa is an all-in-one fluorescence microscope that pairs the highest data quality with remarkably simple day-to-day operation. It easily integrates into any researcher’s toolbox. It becomes a time-efficient, reliable companion for scientists starting to explore time-resolved fluorescence methodologies as well as for experts wanting to push the limits. Truly a microscopy system that everybody can rely on.
Specifications
Excitation
1 - 8 lasers with choice of wavelengths from 375 - 1064 nm
optional: 2 additional exit ports available for coupling additional lasers
Commonly used wavelengths: 405, 440, 485, 532, 560, 595, and 640 nm
Individual laser operation modes : pulsed and continuous wave
(Continuous mode availability depends on the laser model.)
Repetition rates :1 - 40 MHz, for specific wavelengths up to 80 MHz
Laser driver :8 channel Sepia PDL 828L
Motorized main dichroic wheel : 6 positions available for 18 × 25 × 3 mm single, dual, triple, quad-band dichroics
Microscope
inverted Olympus IX73 body (partially motorized)
Objective types: UPLSAPO 60x PlanApochromat, NA 1.2, water immersion, 400 - 900 nm
UPLSAPO 100x PlanApochromat, NA 1.4, oil immersion, 400 - 850 nm
other oil immersion, apochromatic correction, air spaced, IR-enhanced, or long
working distance objectives available
Observation modes : confocal, transmission
optional: epi-widefield illumination, Differential Interferometric Contrast (DIC)
Oculars : Yes
Camera : Optional CCD camera at the left side port
Sample holder : Multi-functional sample holder compatible with several standard sizes of microscope coverslips and petri dishes
96-well plate sample holder
Compatible with cage incubator : Yes
Positional stability
Axial drift (typical values) • For 15-minute measurement: 50 ± 10 nm
• For 2-hour measurement: 100 ± 20 nm
Point Detector Type
• Single Photon Avalanche Diodes (SPAD) for low dark-counts and
maximum detection efficiency
• Hybrid photomultiplier tubes (PMA-Hybrid-40) for optimum timing
performance and when working at high count rates
• 23-pixel SPAD array
Data acquisition
• based on Time-Correlated Single Photon Counting (TCSPC) in Time-
Tagged Time Resolved (TTTR) measurement mode
• simultaneous data acquisition with up to 6 channels
• new dynamic binning format for FLIM imaging
TCSPC Unit : MultiHarp 150 8P
Scanning and positioning
FLIMbee : XY galvo scanner : Min. pixel dwell time: 0.5 µs,
Min. pixel size: 17 nm (60x objective)
Up to 5.2 frames per second for a 512 x 512 pixel image
Max. scan field: 200 x 200 µm (60x objective)
Possibility to bypass the FLIM bee galvo scanner for point measurements
XY objective scanning : Min. pixel dwell time: 0.2 ms
Min. pixel size: 1 nm (independent of objective)
Max. scan field: 80 x 80 µm (independent of objective)
Luminosa software
Operating System : Windows 11
GPU-based programming OpenGL
Context-based workflows for easy
acquisition and analysis
For single-molecule FRET burst analysis, single-molecule imaging, FCS in vitro,
FCS in cells, FLIM, FLIM-FRET, steady-state FRET, anisotropy imaging
Application
-Dynamic structural biology at the single molecule level
-Cellular mechanisms driven by phase separation
-Environmental sensing
-Mapping dynamics and structure of cellular membranes
-Plant development and structure