RMS100X-PFO

• 100X magnification for high-resolution microscopy.
• High numerical aperture (NA) of 1.30 for enhanced light collection and resolution.
• Oil immersion design for high-NA imaging applications.
• The plan fluorite optical design provides excellent correction of spherical and chromatic aberrations.
• Designed for infinity-corrected microscope systems.
• 0.20 mm working distance for high-resolution imaging of samples positioned close to the objective.
• 180 mm design tube lens focal length for use with compatible Olympus-style infinity-corrected systems.
• RMS threading for compatibility with standard microscope objective mounts and adapters.
• Suitable for visible and near-infrared microscopy applications.
• Provides a flat field of view, making it suitable for imaging across a broad image plane.
• Particularly useful for fluorescence microscopy, laser scanning microscopy, and high-resolution biological imaging.

The Thorlabs RMS100X-PFO is a 100X Plan Fluorite oil immersion microscope objective with a high numerical aperture of 1.30. It is designed for infinity-corrected microscopy systems and uses a 180 mm design tube lens focal length.

The Plan Fluorite optical design provides improved correction of spherical and chromatic aberrations compared with conventional achromatic objectives. Thorlabs describes its Plan Fluorite objectives as being corrected at multiple colors and designed to produce flat images across the field of view, making them well suited for demanding imaging applications such as color laser scanning microscopy.

With a 1.30 NA, the RMS100X-PFO provides high light-gathering capability and is particularly useful for applications requiring high spatial resolution and strong signal collection. Because it is an oil immersion objective, immersion oil is required between the objective and the sample or cover glass during operation; using the objective without the appropriate immersion medium will degrade image quality.

The objective has a 0.20 mm working distance and a 49.19 mm overall length, with standard RMS threading for integration into compatible microscope systems.

Its high NA and Plan Fluorite design make the RMS100X-PFO particularly suitable for applications such as fluorescence microscopy, laser scanning microscopy, optical trapping, and single-particle spectroscopy. Thorlabs specifically notes that high-NA Plan Fluorite objectives such as the RMS100X-PFO can be used in optical tweezers systems for trapping and fluorescence observation of particles such as polystyrene beads.

Product Type: Oil Immersion Microscope Objective
Part Number: RMS100X-PFO
Objective Class: Plan Fluorite
Magnification: 100X
Numerical Aperture (NA): 1.30
Working Distance (WD): 0.20 mm
Effective Focal Length (EFL): 1.8 mm
Design Tube Lens Focal Length: 180 mm
Immersion Medium: Oil
Parfocal Length: 45.06 mm
Objective Threading: RMS
Objective Diameter: 24.0 mm
Overall Length: 49.19 mm
Optical Field Number: 26.5
Wavelength Range: Visible to NIR
Cover Glass Thickness: 0.17 mm
Entrance Pupil Diameter: 4.68 mm
Theoretical Resolution: ~0.26 µm @ 550 nm
Optical Design: Infinity Corrected
Manufacturer Compatibility: Olympus-style 180 mm tube lens systems

The RMS100X-PFO belongs to Thorlabs' family of Olympus Plan Fluorite objectives. The objective is designed around a 180 mm tube lens focal length and uses standard RMS threading.

The RMS100X-PFO is suitable for a wide range of high-resolution microscopy and photonics applications, including:

• Fluorescence Microscopy
• Laser Scanning Microscopy
• High-Resolution Optical Imaging
• Biological Imaging
• Cellular Imaging
• Single-Particle Imaging
• Optical Tweezers
• Single-Particle Spectroscopy
• Confocal Microscopy
• Brightfield Microscopy
• Nomarski DIC Microscopy
• High-NA Laser Focusing
• Photophysics Research
• Photonics Research
• Life Science Research
• Advanced Scientific Imaging

The RMS100X-PFO is particularly well suited to applications requiring high magnification, high numerical aperture, and high-quality multicolor imaging, especially when strong light collection and high spatial resolution are important. Its combination of 100X magnification, NA 1.30, oil immersion, and a Plan Fluorite optical design makes it a strong choice for demanding fluorescence and laser-based microscopy applications.