RMS40X-PFO

• 40X magnification for high-resolution microscopy.
• Oil immersion design for advanced imaging applications.
• Plan Fluorite optical design for improved chromatic and spherical aberration correction.
• 0.13 numerical aperture (NA).
• 0.2 mm working distance for close-range high-resolution imaging.
• Designed for infinity-corrected microscope systems.
• Compatible with a 180 mm focal length tube lens.
• 45.06 mm parfocal length for compatibility with standard RMS objective systems.
• RMS (0.800"-36) threading for straightforward integration into compatible microscope systems.
• Visible-to-NIR wavelength coverage, making it suitable for multicolor imaging and laser-based microscopy.
• Suitable for both imaging and laser focusing applications.
• Designed for applications where high-quality optical imaging and oil immersion are required.

The Thorlabs RMS40X-PFO is a 40X Olympus Plan Fluorite oil immersion microscope objective designed for infinity-corrected imaging systems.

As part of Thorlabs' Olympus Plan Fluorite objective family, the RMS40X-PFO uses a Plan Fluorite optical design that provides correction at multiple wavelengths and a flat field of view. These objectives are designed for visible to near-infrared wavelengths and are particularly well suited for color photomicrography and demanding imaging applications.

The objective provides 40X magnification, a 0.13 NA, and a 0.2 mm working distance. The short working distance allows the objective to perform high-resolution imaging close to the specimen, while the oil immersion configuration is intended to improve optical performance by using immersion oil between the objective and cover glass. Thorlabs notes that using an oil immersion objective without oil will degrade image quality.

The RMS40X-PFO is designed around a 180 mm tube lens focal length and has a 45.06 mm parfocal length. Its standard RMS threading makes it compatible with a wide range of microscope objective mounts and adapters.

Plan Fluorite objectives in this family are suitable for both microscopy and laser focusing. Thorlabs specifically identifies the Plan Fluorite objective family as useful for imaging applications and for focusing laser light, with the RMS40X-PFO providing a higher-magnification option within the oil-immersion range.

Product Type: Oil Immersion Microscope Objective
Part Number: RMS40X-PFO
Objective Class: Plan Fluorite
Magnification: 40X
Numerical Aperture (NA): 0.13
Working Distance (WD): 0.2 mm
Design Tube Lens Focal Length: 180 mm
Parfocal Length: 45.06 mm
Immersion Medium: Oil
Objective Threading: RMS (0.800"-36)
Objective Diameter: 24.0 mm
Overall Length: 49.39 mm
Optical Field Number: 26.5
Wavelength Range: Visible to NIR
Cover Glass Thickness: 0.17 mm
Effective Focal Length (EFL): 4.5 mm
Entrance Pupil Diameter: 6.8 mm
Theoretical Resolution: ~0.4 µm @ 550 nm
Optical Design: Infinity Corrected

The RMS40X-PFO specifications above are based on Thorlabs' current Plan Fluorite objective documentation. The objective is listed with 40X magnification, NA 0.13, 0.2 mm WD, 49.39 mm length, and 26.5 optical field number.

The RMS40X-PFO is suitable for a range of microscopy, imaging, and photonics applications, including:

• Fluorescence Microscopy
• High-Resolution Optical Imaging
• Biological Imaging
• Cellular Imaging
• Laser Scanning Microscopy
• Brightfield Microscopy
• DIC Microscopy
• Multicolor Imaging
• Laser Beam Focusing
• Optical Trapping
• Photophysics Research
• Life Science Research
• Photonics Research
• Scientific Imaging Systems
• Microscope-Based Laser Systems

The RMS40X-PFO is particularly useful for applications requiring 40X magnification, plan fluorite optical performance, and oil immersion in an infinity-corrected microscope system. Its visible-to-NIR coating and Plan Fluorite design make it suitable for demanding imaging applications involving multiple wavelengths, while its RMS threading allows integration with standard microscope hardware.