SymPhoTime 64
Point, 2D, and 3D TTTR data acquisition with online preview of FLIM, FCS, time-traces, or TCSPC histograms FLIM, fast FLIM, FLIM-FRET FCS, FCCS, FLCS, PIE-FCS, coincidence correlation, total correlation FRET, PIE-FRET, fluorescence time traces, anisotropy
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Description
The SymPhoTime 64 software package is an integrated solution for data acquisition and analysis using PicoQuant's time-resolved confocal microscope MicroTime 200, MicroTime 100, LSM upgrade kits or TCSPC electronics. Its clearly structured layout and powerful analysis routines allows the user to focus on the results rather than on the data processing. The software is designed for a 64 bit operation system and features a graphical user interface (GUI), which guides the user through all necessary steps for an individual analysis or measurement process. Data dependencies are directly visible in the underlying workspace concept. An integrated scripting language ("STUPSLANG") even puts the user in a position to freely add new analysis procedures or customize existing ones.
Specifications
Supported TCSPC modules : HydraHarp 400, PicoHarp 300, PicoHarp 330, TimeHarp 260, MultiHarp 150, MultiHarp 160, TimeHarp 200 (Data import ONLY!) Routing : 1 to 8 detectors Measurement modes : Single point, multi-point, 2D imaging (XY, XZ, YZ), 3D imaging (XYT), and oscilloscope mode for alignment purposes Measurement previews: FLIM, FCS, FLCS, FCCS, Time Trace, TCSPC histogram Parallel calculation and display of up to 4 different previews Automated Measurements: Z-Stacks, Time Stacks, Image Stitching, Multi-Point Measurements Hardware control: PDL 828 "Sepia II" laser driver E-710, E-725, E-727, and a wide range scanner controller (Physik Instrumente) Shutter of MicroTime 200 Wide-field fluorescence camera IDS uEye USB3 in MicroTime 200
Application
Time-Resolved Fluorescence Fluorescence Lifetime Imaging (FLIM) Phosphorescence Lifetime Imaging (PLIM) Fluorescence Correlation Spectroscopy (FCS) Fluorescence Lifetime Correlation Spectroscopy (FLCS) Foerster Resonance Energy Transfer (FRET) Stimulated Emission Depletion Microscopy (STED) Dual Focus Fluorescence Correlation Spectroscopy (2fFCS) Pulsed Interleaved Excitation (PIE) Single Molecule Spectroscopy / Detection Pattern Matching Analysis Time-Resolved Photoluminescence (TRPL) TRPL Imaging Lanthanide Upconversion Antibunching