Dianthus uHTS

• Ultra-high-throughput biophysical screening in a 1536-well plate format

• Screen up to 1,000,000 compounds in 5 days

• Measures a full 1536-well plate in less than 10 minutes

• Requires as little as 7 µL per well in 1536-well plates

• Detects target concentrations down to 250 pM

• Measures binding affinity from millimolar to sub-nanomolar range

• Directly measures target engagement without requiring prior knowledge of the binding site

• Uses Spectral Shift technology for rapid, in-solution affinity measurements

• Suitable for challenging targets including GPCRs, kinases, membrane proteins, and ion channels

• Supports discovery of orthosteric, allosteric, and cryptic-site binders

• Designed for walk-away automation and integration with existing laboratory automation

• Provides structured, human- and machine-readable JSON data exports for downstream analysis and data pipelines

Dianthus uHTS™ is an ultra-high-throughput biophysical screening platform designed for large-scale compound library screening and direct target engagement analysis. Using Spectral Shift technology, the system measures molecular binding directly in solution without requiring surface immobilization or prior knowledge of the target's binding site. The 1536-well format enables researchers to screen hundreds of thousands to millions of compounds at a scale suitable for primary high-throughput screening campaigns. Dianthus uHTS can generate direct binding data for challenging targets, including membrane proteins, GPCRs, ion channels, and kinases, while supporting the discovery of both known and previously unidentified binding sites. The platform can operate as a standalone instrument or integrate with existing laboratory automation through a gRPC framework, enabling hands-free, high-throughput workflows.

Measurement technology: Spectral Shift Measurement principle: In-solution binding affinity / target engagement Plate format: 1536-well SBS format Throughput: 1536 data points in <10 minutes Large-scale screening: Up to 1,000,000 compounds in 5 days Sample volume: As low as 7 µL/well (1536-well); 9 µL/well (384-well) Minimum target concentration: Down to 250 pM Affinity range: Millimolar to sub-nanomolar Detection wavelengths: 650 nm and 670 nm Excitation wavelength: 590 nm Measurement environment: Isothermal Measurement type: Non-destructive Binding information: Direct target engagement and K_d Automation: gRPC-based integration with laboratory automation Operation: Standalone or automated workflow Data export: CSV, XLSX, JSON Data integration: Open and FAIR APIs

• Ultra-high-throughput screening (uHTS)

• High-throughput screening (HTS)

• Primary compound screening

• Fragment screening

• Hit identification

• Hit validation

• Hit-to-lead optimization

• Direct target engagement

• Drug discovery

• Kinase screening

• GPCR screening

• Membrane protein screening

• Ion channel research

• Allosteric binder discovery

• Cryptic binding-site discovery

• Orthosteric binding studies

• Molecular glue discovery

• Targeted protein degradation (TPD) research

• Challenging target characterization